Document Type : Original Article

Authors

1 Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari Iran

2 Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran Iran

3 Thalassemia Research Center, Mazandaran University of Medical Sciences, Sari Iran

Abstract

Background: Curcumin is a natural polyphenolic material with antioxidative, anti-inflammatory, and anticancer effects. In this study, we attempted to assay anti proliferative and apoptotic properties of polymeric micelles of curcumin on two colorectal cancer cell lines and normal human fibroblast cells.
Method: In this experimental study, cancer cells HT29, HCT116 and normal human fibroblast cells (HGF) were subjected to concentrations of Nano- curcumin (1, 50, 100, 250, and 500 µg/ml). After incubation for 48 hours, cell viability was assessed with "MTT"(3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. Annexin V-FITC and Propidium iodide staining were done with flow cytometry for evaluation of apoptosis. The results were shown as mean ± standard deviation. Statistical significance was assessed utilizing ANOVA and Dunnetts t-test (P < 0.01).
Results: According to MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay results, IC50 value of Nano- curcumin in HT29, HCT116, and HGF were 70.63, 123.9, and 168.53 µg/ml, respectively. We also discovered that Nano-curcumin can make indicative apoptosis in cancer cells, which could be compared with cisplatin<0.01.
Conclusion: These results revealed remarkable anti-proliferative and apoptotic effects of polymeric Nano-micelles of curcumin in colorectal cancer cell lines.

Keywords

This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination, and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi:10.30476/mejc.2021.87699.1429