TY - JOUR ID - 47395 TI - Evaluation of Curcumin Nano-micelle on Proliferation and Apoptosis of HT29 and Hct116 Colon Cancer Cell Lines JO - Middle East Journal of Cancer JA - MEJC LA - en SN - 2008-6709 AU - Mohammadi, Hamidreza AU - Shokrzadeh, Mohammad AU - Akbari-Dafsari, Omid AU - Amani, Nahid AU - Modanlou, Mona AU - Mohammadpour, Abbas AU - Jafari-Sabet, Majid AU - Ataee, Ramin AD - Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari Iran AD - Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran Iran Y1 - 2022 PY - 2022 VL - 13 IS - 1 SP - 99 EP - 109 KW - Colonic neoplasms KW - Cell Proliferation KW - apoptosis KW - Curcumin DO - 10.30476/mejc.2021.87699.1429 N2 - Background: Curcumin is a natural polyphenolic material with antioxidative, anti-inflammatory, and anticancer effects. In this study, we attempted to assay antiproliferative and apoptotic properties of polymeric micelles of curcumin on two colorectal cancer cell lines and normal human fibroblast cells.Method: In this experimental study, cancer cells HT29, HCT116 and normal human fibroblast cells (HGF) were subjected to concentrations of Nano- curcumin (1, 50, 100, 250, and 500 μg/ml). After incubation for 48 hours, cell viability was assessed with "MTT"(3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. Annexin V-FITC and Propidium iodide staining were done with flow cytometry for evaluation of apoptosis. The results were shown as mean ± standard deviation. Statistical significance was assessed utilizing ANOVA and Dunnetts t-test (P < 0.01).Results: According to MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay results, IC50 value of Nano- curcumin in HT29, HCT116, and HGF were 70.63, 123.9, and 168.53 μg/ml, respectively. We also discovered that Nano-curcumin can make indicative apoptosis in cancer cells, which could be compared with cisplatin <0.01.Conclusion: These results revealed remarkable antiproliferative and apoptotic effects of polymeric Nano-micelles of curcumin in colorectal cancer cell lines. UR - https://mejc.sums.ac.ir/article_47395.html L1 - https://mejc.sums.ac.ir/article_47395_98be79ace020c3dfc6a461a9ac063853.pdf ER -