Department of Biochemistry, University of Madras, Chennai, Tamil Nadu, India
Abstract
Background: Colorectal cancer, a major health concern worldwide, is the third most common form of cancer and second leading cause of cancer-related deaths. The flavonoids are naturally occurring diphenylpropanoids ubiquitous in plant foods and important components of the human diet. Luteolin, a bioflavonoid, possesses many beneficial effects including antioxidant, anti-inflammatory, anti-allergic activities. Methods:We used the HCT-15 colon adenocarcinoma cell line in this study. Cells were treated with luteolin (100 µM).Results: Membrane damage markers such as alkaline phosphatase and lactate dehydrogenase were analyzed in a time-dependent manner. Luteolin increased reactive oxygen species in a time-dependent manner. DNA damage, a hallmark of apoptosis, was induced by luteolin as analyzed by agarose gel electrophoresis.Conclusion: Luteolin acts as a potential cytotoxic agent that can be used to treat colorectal cancer.
Pandurangan, A. Kumar & Ganapasam, S. (2013). Cytotoxic Effect of Luteolin on Human Colorectal Cancer Cell Line (HCT-15): Crucial Involvement of Reactive Oxygen Species. Middle East Journal of Cancer, 4(4), 175–180.
MLA
Pandurangan, A. Kumar, & Ganapasam, S. "Cytotoxic Effect of Luteolin on Human Colorectal Cancer Cell Line (HCT-15): Crucial Involvement of Reactive Oxygen Species", Middle East Journal of Cancer, 4, 4, 2013, 175–180.
HARVARD
Pandurangan, A. Kumar, & Ganapasam, S. (2013). 'Cytotoxic Effect of Luteolin on Human Colorectal Cancer Cell Line (HCT-15): Crucial Involvement of Reactive Oxygen Species', Middle East Journal of Cancer, 4(4), pp. 175–180.
CHICAGO
Pandurangan, A. Kumar & Ganapasam, S., "Cytotoxic Effect of Luteolin on Human Colorectal Cancer Cell Line (HCT-15): Crucial Involvement of Reactive Oxygen Species." Middle East Journal of Cancer, 4 4 (2013): 175–180,
VANCOUVER
Pandurangan A. Kumar, Ganapasam S. Cytotoxic Effect of Luteolin on Human Colorectal Cancer Cell Line (HCT-15): Crucial Involvement of Reactive Oxygen Species. Middle East Journal of Cancer. 2013;4(4):175–180.
