Document Type: Original Article

Authors

1 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

2 Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Medical Radiation Sciences Research Group, Tabriz University of Medical Sciences, Tabriz, Iran

4 Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

5 Department of Cancer and Inflammation Research, University of Southern Denmark, Odense, Denmark

Abstract

Background: MicroRNAs are involved in response to therapeutic agents and have the ability to regulate the expression level of the targets associated with cancer growth and progression. As a dangerous signal in tumor cells, increased expression level of MHC class I chain-related protein A (MICA) could activate the immune system and induce responses to tumor cells. We conducted the present research to study the effect of linoleic acid (LA) and docetaxel alone or in combination with miR-106b, miR-20a, and MICA expression level in metastatic gastric cancer (GC) cell line, MKN-45.
Method: The study was an in vitro study using the gastric cancer cell line MKN-45, which was cultured and treated with docetaxel and LA. Subsequently, the expression level of miR-106b, miR-20a, and MICA were assessed with quantitative real-time PCR.
Results: MiR-106b decreased in LA and LA/docetaxel (p p =0.002), and increased in docetaxel alone (p =0.01). Meanwhile, miR-20a significantly decreased in docetaxel and LA/docetaxel (p <0.0001), increased in LA treatment (p =0.02). Regarding MICA, it significantly decreased in all the treated cells (p <0.0001, p <0.0001, and p =0.0002 for docetaxel, LA and docetaxel/LA, respectively) but with different reduction intensities.
Conclusion: Using LA or docetaxel alone had a different effect on miR-106b, miR-20a, and MICA expression level, yet in a simultaneous treatment, their positive effects were intensified. LA enhanced the effect of docetaxel concerning the expression level of miR-106b, miR-20a, and MICA and vice versa, which suggested that LA could be employed as an effective complementary agent in GC along with docetaxel.

Keywords

This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination, and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi:10.30476/mejc.2021.87303.1406