Document Type: Original Article


1 Molecular Pathology and Cytogenetic Section, Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

2 Molecular Pathology and Cytogenetic Section, Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

3 Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran


Background: Given the prognostic importance of cytogenetic aberrations in plasma cell neoplasms, the present retrospective study was conducted to analyze cytogenetic abnormalities in plasma cell myeloma cases in a single center in the Middle East.
Method: In this retrospective cross-sectional study, we selected 42 patients referred to the molecular and cytogenetic department from 2013 to 2016 for initial assessment by immunohistochemical, flow cytometric, and cytogenetic studies. Chromosomal analysis was performed after a 72-hour unsynchronized culture and Giemsa banding; the result was reported according to ISCN 2016.
Results: 32.5% of the patients showed an abnormal karyotype, of whom 53.8% were hyperdiploid and the rest were assigned to the non-hyperdiploid group. The gain of 1q and monosomy 13 / deletion 13q was the most common structural abnormalities accounting for 38.4% and 30.7%, respectively. t (11;14) was the only detected 14q32 rearrangement observed in 15.4% of the cases. The mean survival time in normal, hyperdiploid, and non-hyperdiploid groups were 29.5±1.7, 16.6±2.9 and 6.1±2.1 months, respectively.
Conclusion: Cytogenetic abnormalities of plasma cell myeloma in this center were relatively similar to previous reports in the literature; moreover, hyperdiploidy was the most common cytogenetic aberration. Therefore, we recommend the use of more precise techniques such as FISH in addition to conventional G banding to detect cryptic aberrations. Survival of the non-hyperdiploid group was the worst.


This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination, and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi:10.30476/mejc.2020.83285.1158