Document Type : Original Article(s)
Authors
- Ehab Maher 1
- Gamalat Gedawy 2
- Walid Fathy 3
- Sabah Farouk 1
- Ahmed Abd El Maksoud 4
- Adel A. Guirgis 1
- Hany Khalil 1
1 Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt
2 Department of Clinical Biochemistry and Molecular Diagnostic, National Liver Institute, Menofyia University, Sebin El-Kom, Egypt
3 Department of Clinical Pathology, Faculty of Medicine, Menofyia University, Sebin El-Kom, Egypt
4 Industrial Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt
Abstract
Background: Colorectal cancer (CRC), caused by abnormal cells growing in the colon or rectum, has a high mortality rate worldwide. On the other hand, microRNAs are small non-coding RNAs that contain approximately 22 nucleotides in length. They are upregulated in a wide range of human cancers such as CRC. MiRNA-21 post-transcriptionally regulates the expression of many tumor suppressor genes such as P53 gene. This indicates that miRNA-21 interacts like oncogenes and is required for CRC development.
Method: The current original study was conducted in the National Liver Institute, Menofyia University, Egypt. We collected a total of 40 blood samples from CRC patients 40 samples from healthy individuals who served as controls. Quantitative real-time PCR detected the levels of miRNA-21 and the fold changes of phosphates-tensin homology (PTEN) gene expression, as a tumor suppressor gene, in blood samples.
Results: The expression levels of miR-21 were upregulated in all obtained samples from patients with CRC in association with aging, gender, and tumor-node-metastasis staging. Furthermore, patients with poor and well-differentiated CRC revealed reduced levels of PTEN gene expression. We observed a putative binding site of miR-21 in PTEN gene sequences. This indicates the direct cleavage between miR-21 and PTEN coding sequence. Prediction analysis for other potential targets identified several malignancy factors and tumor suppressor genes with putative seeding regions for miR-21 such as STAT3, transforming growth factor-beta, tumor necrosis factor-α (TNF-α), and programmed cell death CD4.
Conclusion: The current data exhibited the potential dual role of hsa-miR-21 in regulating cancer progression and showed that hsa-miR-21 is an efficacious biomarker for CRC d evelopment and an attractive candidate for CRC treatment during early transformation.
Keywords
How to cite this article:
Maher E, Gedawy G, Fathy W, Farouk S, Maksoud AA, Guirgis AA, et al. Hsa-miR-21-mediated cell death and tumor metastases: A potential dual response during colorectal cancer development. Middle East J Cancer. 2020;11(4): 483-92. doi: 10.30476/mejc.2020. 83146.1139.