Middle East Journal of Cancer

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Editorial Board

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

News

Forms

Journal Metrics

A Novel Combination of Pterostilbene and Dexamethasone Enhances Anti-proliferation and Apoptosis Induction Properties in Lymphoblastic Leukemia Cell Line

Document Type : Original Article(s)

Authors

1 Medical Plant Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

2 Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

3 Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

4 Pathology and Hematology Department, Shahrekord University of Medical Sciences, Shahrekord, Iran

Abstract

Background: T-cell acute lymphoblastic leukemia is an aggressive hematologic malignancy that results from the transformation of T-cell progenitors. Despite the significant advances in the current treatments, the side-effects of conventional chemotherapy regimens are still a major concern. Pterostilbene (PT) is a natural compound reported to have antitumor effects. This study aimed to investigate the effect of PT combined with dexamethasone on the proliferation inhibition and apoptosis stimulation in a lymphoblastic leukemia cell line.
Methods: In this experimental study, we cultured Jurkat cell line in RPMI1640 culture medium under standard conditions. We incubated the cells with different concentrations of PT and dexamethasone, separately or in combination for 48 h. MTS assay investigated the cell viability. We assessed apoptosis induction by annexin V-FITC/PI and flow cytometry analysis.
Results: PT and dexamethasone reduced the viability of the cells with inhibition concentrations of 60.97±3.36 and 451.1±10.1 μM, respectively, in 48 h. None of the concentrations of dexamethasone, employed alone, significantly reduced the cell viability. The combination of 450 μM dexamethasone with 60 μM PT induced apoptosis in more than 70% of the cells with a significant difference compared to control.
Conclusion: PT increased the antiproliferative and apoptosis-inducing activity of dexamethasone in Jurkat cell line. This combination drug strategy can be a novel approach for a more powerful anticancer therapy.

Keywords

Full Text

How to cite this article:

Sourani Z, Rezaei Dezaki Z, Rahimnejad T, Pourgheysari B. A novel combination of pterostilbene and dexamethasone enhances anti-proliferation and apoptosis induction properties in lymphoblastic leukemia cell line. Middle East J Cancer. 2020; 11(4):469-75. doi: 10.30476/ mejc.2020.82206.1067.

 

References
1.       Gökbuget N, Stanze D, Beck J, Diedrich H, Horst H-A, Hüttmann A, et al. Outcome of relapsed adult lymphoblastic leukemia depends on response to salvage chemotherapy, prognostic factors, and performance of stem cell transplantation. Blood. 2012;120(10):2032-41.
2.       Belver L, Ferrando A. The genetics and mechanisms of T cell acute lymphoblastic leukaemia. Nat Rev Cancer. 2016;16(8):494-507. doi: 10.1038/nrc.2016.63.
3.       Roti G, Stegmaier K. New Approaches to Target T-ALL. Front Oncol. 2014;4:170. doi: 10.3389/fonc.2014.00170.
4.       Senthilkumar R, Chen BA, Cai XH, Fu R. Anticancer and multidrug-resistance reversing potential of traditional medicinal plants and their bioactive compounds in leukemia cell lines. Chin J Nat Med. 2014;12(12):881-94. doi: 10.1016/S1875-5364(14)60131-X.
5.       Asgari S, Rafieian-Kopaei M, Pourgheysari B, Ansari Samani R, Deris F, Shahinfard N, et al. Allium hirtifolium Boiss: Radical scavenging property and the lowering effects on blood fibrinogen and factor VII. Life Science Journal. 2012;9(3):1793-8.
6.       Sourani Z, Pourgheysari B, Beshkar P, Shirzad H, Shirzad M. Gallic acid inhibits proliferation and induces apoptosis in lymphoblastic leukemia cell line (C121). Iran J Med Sci. 2016;41(6):525-530.
7.       Anantharaju PG, Gowda PC, Vimalambike MG, Madhunapantula SV. An overview on the role of dietary phenolics for the treatment of cancers. Nutr J. 2016;15(1):99.
8.       Athar M, Back JH, Kopelovich L, Bickers DR, Kim AL. Multiple molecular targets of resveratrol: Anti-carcinogenic mechanisms. Arch Biochem Biophys. 2009;486(2):95-102.
9.       Burns J, Yokota T, Ashihara H, Lean ME, Crozier A. Plant foods and herbal sources of resveratrol. J Agric Food Chem.2002;50(11):3337-40.
10.     Kapetanovic IM, Muzzio M, Huang Z, Thompson TN, McCormick DL. Pharmacokinetics, oral bioavailability, and metabolic profile of resveratrol and its dimethylether analog, pterostilbene, in rats. Cancer Chemother Pharmacol. 2011;68(3):593-601.
11.     Hasiah A, Ghazali AR, Weber J, Velu S, Thomas N, Inayat Hussain S. Cytotoxic and antioxidant effects of methoxylated stilbene analogues on HepG2 hepatoma and Chang liver cells: Implications for structure activity relationship. Hum Exp Toxicol. 2011;30(2):138-44.
12.     Moon D, McCormack D, McDonald D, McFadden D. Pterostilbene induces mitochondrially derived apoptosis in breast cancer cells in vitro. J Surg Res. 2013;180(2):208-15.
13.     Schneider JG, Alosi JA, McDonald DE, McFadden DW. Pterostilbene inhibits lung cancer through induction of Apoptosis1. J Surg Res. 2010;161(1):18-22.
14.     Pei HL, Mu DM, Zhang B. Anticancer activity of pterostilbene in human ovarian cancer cell lines. Med Sci Monit. 2017;23:3192-3199.
15.     Ringler I, West K, Dulin W, Boland EW. Biological potencies of chemically modified adrenocorticosteroids in rat and man. Metabolism. 1964;13(1):37-44.
16.     Ghasemi-Pirbaluti M, Pourgheysari B, Motaghi E, Askarian Dehkordi N, Surani Z, Shirzad M, et al. Effect of Epigallocatechin-3-gallate (EGCG) on cell proliferation inhibition and apoptosis induction in lymphoblastic leukemia cell line. Journal of HerbMed Pharmacology. 2015;4.
17.     Wang Y, Ding L, Wang X, Zhang J, Han W, Feng L, et al. Pterostilbene simultaneously induces apoptosis, cell cycle arrest and cyto-protective autophagy in breast cancer cells. Am J Transl Res.2012;4(1):44.
18.     Roslie H, Chan KM, Rajab NF, Velu SS, Abd SAIAS, Bunyamin I, et al. 3, 5-Dibenzyloxy-4’-hydroxystilbene induces early caspase-9 activation during apoptosis in human K562 chronic myelogenous leukemia cells. J HerbMed Pharmacol. 2012;37(1):13-21.
19.     Mena S, Rodríguez ML, Ponsoda X, Estrela JM, Jäättela M, Ortega AL. Pterostilbene-induced tumor cytotoxicity: a lysosomal membrane permeabilization-dependent mechanism. PLoS One. 2012;7(9):e44524. doi: 10.1371/journal.pone.0044524.
20.     Tolomeo M, Grimaudo S, Di Cristina A, Roberti M, Pizzirani D, Meli M, et al. Pterostilbene and 3′-hydroxypterostilbene are effective apoptosis-inducing agents in MDR and BCR-ABL-expressing leukemia cells. Int J Biochem Cell Biol. 2005;37(8):1709-26.
21.       Mannal P, McDonald D, McFadden D. Pterostilbene and tamoxifen show an additive effect against breast cancer in vitro. Am J Surg. 2010;200(5):577-80.
22.     Soltani A, Pourgheysari B, Shirzad H, Sourani Z. Antiproliferative and apoptosis-inducing activities of thymoquinone in lymphoblastic leukemia cell line. Indian J Hematol Blood Transfus.2017;33(4):516-24.
23.     Sourani Z, Shirzad H, Shirzad M, Pourgheysari B. Interaction between Gallic acid and Asparaginase to potentiate anti-proliferative effect on lymphoblastic leukemia cell line. Biomed Pharmacother. 2017; 96:1045-54.
Middle East Journal of Cancer
Volume 11, Issue 4 - Serial Number 44
October 2020
Pages 469-475
Files
Share
How to cite
Statistics