Document Type : Original Article(s)

Authors

• Narjes Soltani ¹
• Ehsan Ghayoor Karimiani ²
• Mohammadreza Farzanehfar ³
• Baratali Mashkani ⁴
• Amirhossein Jafarian ⁵
• Hami Ashraf ⁶
• Arash Akhavan Rezyat ⁷
• Mohammad Soukhtanloo ⁴

¹ Cancer Molecular Pathology Research Center, Ghaem Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

² Hope Genetic Polyclinic, Mashhad, Iran

³ Department of Internal Medicine, Ghaem Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

⁴ Department of Medical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

⁵ Cancer Molecular Pathology Research Center, Department of Pathology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

⁶ Razavi Cancer Research Center, Research and Education Department, Razavi Hospital, Mashhad, Iran

⁷ Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Background: Colorectal cancer, the third most common type of cancer is a major cause of mortality worldwide. If colorectal cancer is detected at the early stages, the 5-year survival rate is 90%. MEIS1 homeobox gene is implicated in numerous solid tumors and hematological malignancies. Therefore, the present study aims to investigate the methylation status of the MEIS1 gene in colorectal cancer.Methods: We used real-time quantitative methylation-specific PCR to detect MEIS1 promoter methylation in 42 colorectal cancer tissues and 42 normal colorectal tissues.Results: Methylation was observed only in the positive control samples - CG Genome Universal Unmethylated DNA and CG Genome Universal Methylated DNA. There was no change observed in MEIS1 promoter methylation status in 42 patients.Conclusion: The results of the current study indicated that the MEIS1 gene promoter was not methylated in the cases. Gene expression study confirmed the unmethylated status of the MEIS1 gene in the colorectal cancer process among the studied population.