Document Type : Original Article(s)
Authors
1 Department of Biology, Am.C Islamic Azad University, Amol, Iran
2 Medicinal Plants Research Center, Mazandaran University of Medical Sciences, Sari Iran
3 Department of Medical Biotechnology, Faculty of Advanced Technologist in Medicine, Mazandaran University of Medical Sciences, Sari Iran
Abstract
Background: Serotonin plays a proliferative role by stimulating the cAMP-dependent mitogen activated protein kinase pathway. Also, there is some evidence about the role of 5HT (5-Hydroxytryptamine) receptors in some cancers such as gastrointestinal cancers.
Therefore, we have aimed to investigate the effect of 5HT2A receptor antagonist (ritanserin) on the growth of tumor and expression of apoptotic and angiogenesis factors as (Cyclooxygenase (COX2) and epidermal growth factor (EGFR)) in an in-vivo model in nude mice.
Method: In this experimental study, Ritanserin (5mg/kg, 0.05 cc, IP) was injected into mice for 21 days, and intradermal tumor induced by injecting 10,000,000 (HT29) suspension of colorectal cells into the flank muscle of nude mice and cisplatin used as positive control. Tumor size was examined macroscopically three times a week and angiogenic genes as EGFR and COX2 expressions were evaluated by real-time PCR. Statistical analysis was performed using SPSS software version 22 with ANOVA test.
Results: Tumor size in the Ritanserine group significantly decreased as compared with the control group (P < 0.05). Expression of COX2 was increased in the cisplatin group as compared with the control group (P < 0.05). Also, ritanserin increased COX2 expression as compared with the control group (P < 0.05), while this effect was more in the cisplatin-ritanserine group. Moreover, the expression of EGFR increased by ritanserine (P <0.05) and the combination of ritanserine and cisplatine shows a synergic effect.
Conclusion: Our study indicates that Ritanserin, as a 5HT2A receptor antagonist, has an antitumor and antiangiogenic effect in the xenograft model in nude mice. The effect of ritanserin is partly due to the inhibition of COX2, as an inflammatory factor, and EGFR, as an angiogenic factor.
Highlights
Esamil Fattahi (google scholar)
Keywords
Main Subjects
Please cite this article as: Hosseinzadeh F, Ataee R, Fattahi E, Ghalhenoi H. The inhibitory effect of Ritanserin (5ht2 receptor antagonist) on growth and angiogenesis in model of ht29 colon cancer cells incubation in nude mice. Middle East J Cancer. 2026: in press. doi: 10.30476/mejc.2026.106688.2267.
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