Document Type : Original Article(s)
Authors
- Ehsan Zare Mehrjardi 1
- Shadi Montazerolhojeh 2
- Masoud Dehghan Tezerjani 3
- Safieh Dehshiri 2
- Mahmood Dehghani Ashkezari 2
- Seyed Morteza Seifati 2
1 Department of Industrial Environmental and Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
2 Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran
3 Abortion Research Centre, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Abstract
Background: Breast cancer is a multifactorial malignancy with uncertain treatment outcomes that make its challenging treatment. Balanced level of genes expression, including BAX, BCL2L1 (bcl-xl) and BCL-2 are needed to regulate apoptosis pathway in cancers. The aim of this study was to examine the impact of flaxseed extract on the expression of BAX, BCL-2, and BCL2L1 (bcl-xl) gens in BT-474 and MCF-7 breast cancer cells.
Method: This in vitro experimental study investigated the impact of flaxseed extract on apoptosis and the proliferation of BT-474 and MCF-7 breast cancer cells. MTT assay was conducted on flaxseed extract treated BT-474 and MCF-7 cells at concentrations 3.90628, 7.8125, 156.125, 312.5, 125, 2, 1000, and 2000 µg/ml for 24, 48 and 72h. IC50 (μg/ml) was calculated for flaxseed extract in both cells. The expression of BCL2L1 (bcl-xl), bcl-2 and Bax genes were evaluated at these concentrations using real time Polymerase Chain Reaction (PCR). The data from different groups were analyzed using the Student's t-test.
Results: Flaxseed extract reduced the multiplication and growth of BT-474 and MCF-7 cells in a concentration-dependent manner over 24, 48, and 72 hours of incubation. In both flaxseed-extract exposed BT-474 and MCF-7 cell lines, the BAX gene expression increased (P < 0.05); however, the gene expression of BCL2L1 (bcl-xl) and BCL-2 genes decreased (P < 0.05).
Conclusion: Incubating BT-474 and MCF-7 with flaxseed extract repressed their growth and induced apoptosis. These results may provide precious information for developing a plant-based agent to prevent or treat breast cancer.
Highlights
Ehsan Zare Mehrjardi (PubMed)
Seyed Morteza Seifati (Google Scholar)
Keywords
Main Subjects
This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination, and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.30476/mejc.2024.102754.2108
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