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Evaluation of Potential miR-221, miR-93, miR-21, and miR-24 as Molecular Markers for Laryngeal Squamous Cell Carcinoma

Articles in Press

Document Type : Original Article(s)

Authors

1 Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

2 Department of Basic Oncology, Ege University, Institute of Health Sciences, Izmir, Turkey

3 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

4 Otolaryngology Head and Neck surgery department, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

10.30476/mejc.2024.102700.2105

Abstract

Background: Laryngeal cancer, the most prevalent head and neck malignancy, represents 2.4% of all tumors and is the 11th most common tumor worldwide. Laryngeal squamous cell carcinoma (LSCC) includes 85-90% of all laryngeal tumors and despite significant progression in screening and diagnostic approaches, occurrence and mortality has not reduced in recent decade. According to recent evidence, microRNA-221(miR-221), miR-93, miR-21, and miR-24 have possible roles in the carcinogenesis process of LSCC. In this study, we aimed to investigate any considerable changes in the micro-RNAs (miR) expression, and whether they have the potential to be a prognostic or diagnostic biomarker for LSCC.
Method: This case-control study examined the expression level of target micro-RNAs in 30 LSCC and their matched marginal healthy samples. The expression level of genes and their relationship with the patient’s clinicopathological characteristics were analyzed using GraphPad Prism v.6.00, with paired t-tests and receiver operating characteristics (ROC) curve tests. A P value less than 0.05 was considered statistically significant.
Results: miR-24 is considerably down regulated in cancer tissues (Fold change = 0.89, P = 0.0213) while miR-21 (fold change: 1.16, P = 0.0063), miR-93 (Fold change: 1.123, P = 0.0448) and miR-221 (Fold change: 1.313, P< 0.0001) were upregulated in LSCC tumor tissue. Among these microRNAs, only mir-221 has related to clinicopathological features and has an acceptable ROC area (0.7860), suggesting its potential as a diagnostic marker.
Conclusion: The results of this study revealed that miR-221 have moderate diagnostic accuracy and could be used as potential biomarkers in LSCC. Additional combination and confirmation with other biomarkers are suggested.

Highlights

Habib Azimi (PubMed)

Shahram Ghasembaglou (Google Scholar)

Keywords

Main Subjects

This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination, and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.30476/mejc.2024.102700.2105

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Middle East Journal of Cancer

Articles in Press, Accepted Manuscript
Available Online from 23 November 2024
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