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High Expression of Myosin ⅩⅥ Predicts Poor Prognosis in Head and Neck Squamous Cell Carcinoma

Articles in Press

Document Type : Original Article(s)

Authors

1 Saveetha Medical College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, India

2 Molecular Biology Lab, Centre for Cellular and Molecular Research, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, India

3 Clinical Genetics Lab, Centre for Cellular and Molecular Research, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, India

4 Department of Oral Pathology and Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, India

10.30476/mejc.2024.101434.2026

Abstract

Background: Myosins, a superfamily of actin-dependent molecular motors, have emerged as crucial players in tumorigenesis. This study investigates the role of Myosin ⅩⅥ (MYO16), an unconventional myosin, in HNSCC.
Method: In this case-control study, we employed multiple databases to investigate the expression of MYO16 in samples from the cancer genome atlas (TCGA), focusing on head and neck squamous cell carcinoma (HNSCC) along with associated clinicopathological features. Since HNSCC primarily includes oral squamous cell carcinoma (OSCC), we additionally validated the mRNA level of MYO16 in OSCC samples using the real-time quantitative polymerase chain reaction (RT-qPCR) method. Moreover, we used various online databases to uncover the relationship between MYO16 and tumor infiltration. Statistical analysis was performed using GraphPad Prism, and the significance was determined with Student's t-test.
Results: Comprehensive analyses across diverse databases consistently reveal a significant upregulation of MYO16 expression in HNSCC. RT-qPCR analysis revealed that MYO16 is significantly up-regulated in OSCC tumor tissue samples. Correlation with clinicopathological features and survival analysis underscores its potential prognostic value. Furthermore, MYO16 interactions with immune cells within the tumor microenvironment are negatively associated with immune genes.
Conclusion: This study identifies MYO16 as a potential biomarker associated with HNSCC development, and emphasizes its significance as a potential therapeutic target, aligning with its diverse roles across cellular processes. Further experimental studies are necessary to elucidate MYO16 functional implications and clinical relevance in HNSCC.

Highlights

Paramasivam Arumugam (Google Scholar)

Keywords

Main Subjects

This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination, and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.30476/mejc.2024.101434.2026

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Middle East Journal of Cancer

Articles in Press, Accepted Manuscript
Available Online from 13 July 2024
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