Middle East Journal of Cancer

Evaluation of Fis-1 and miR-484 Expression Levels in Tumor Tissue Samples and Healthy Tumor Margins in Lung Cancer

Document Type : Original Article(s)

Authors

Leila Namvar 1
Mehdi Nadiri 1
Zahra Mohebbi 1
Milad Asadi 2
Maryam Seyyedi 1
Amir Amiri-Sadeghan 1
Zahra Soleimani 3
Shahriyar Hashemzadeh 1
Yeganeh Khalili 1
Habib Zarredar 1

1 Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

2 Department of Basic Oncology, Ege University, Institute of Health Sciences, Izmir, Turkey

3 Rahat Breathe and Sleep Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

https://doi.org/10.30476/mejc.2023.96826.1839
Abstract
Background: MicroRNAs (miRNAs) regulate gene expression and various cellular activities. They also hold significant importance in the progression and development of human malignancies. Among these, miRNA-484 and the Fis-1 gene have been identified as having substantial roles in lung cancer. This study aims to ascertain miRNA-484 and Fis-1 gene expression levels in non-small cell lung cancer (NSCLC) patients.
Method: In this case-control study, 45 pairs of tumor tissues and their corresponding healthy margin tissues were surgically obtained from NSCLC patients and promptly preserved in liquid nitrogen after excision. Total RNA extraction was performed using TRIzol, followed by cDNA synthesis using a designated kit. Afterward, we used quantitative reverse transcription polymerase chain reaction (qRT-PCR) to measure the expression levels of miRNA-484 and the Fis-1 gene. Furthermore, the clinicopathological characteristics of the NSCLC patients were assessed.
Results: Our findings revealed an upregulation of miRNA-484 expression and downregulation of Fis-1 gene expression in NSCLC tissues compared with non-tumor tissues. Additionally, significant correlations were observed between miRNA-484 and Fis-1 gene expression levels and clinicopathological features of the patients, including factors such as lymph node involvement and distant metastasis.
Conclusion: These findings suggest the potential utility of Fis-1 and miR-484 as prognostic and diagnostic markers in NSCLC.

Highlights

Leila Namvar (Google Scholar)

Habib Zarredar (Google Scholar)

Keywords

miRNA-486
Fis-1 protein
Carcinoma
Non-small-cell lung
Malignancy
Subjects

How to cite this article:

Namvar L, Nadiri M, Mohebbi Z, Asadi M, Seyyedi M, Amiri-Sadeghan A, et al. Evaluation of Fis-1 and miR-484 expression levels in tumor tissue samples and healthy tumor margins in lung cancer. Middle East J Cancer. 2024;15(1):1-7. doi: 10.30476/mejc.2023. 96826.1839.

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