Document Type : Original Article

Authors

1 Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt

2 Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

3 Department of Animal Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt

4 Department of Chemistry, Faculty of Science, Kafrelsheikh University, Kafrelsheikh, Egypt

Abstract

Background: Cancer is a disease in which molecular changes of the growth factors and the relevant signaling cause uncontrolled growth and division of cells. The most common factors involved in cancer initiation and development include epidermal growth factor, mitogen-activated protein kinase, and autophagy effectors.
Method: This expermintal study was conducted to investigate the potential anticancer properties of a number of agents, including interferon-gamma, rapamycin, and vitamin B17, which were compared to Sorafenib in hepatucellular carcinoma HepG2 cell line and stem cells. Cells were cultured in RPMI medium with 10% fetal bovine serum, 4 mM sodium pyruvate, 4 mM L-glutamine, and 100 U/mL penicillin/streptomycin. Cell viability and levels of lactate dehydrogenase were investigated for the cytotoxic potential of these agents in both kinds of cells. The expression profile of Raf-1, autophagy-related LC3B, TP53, caspase 3 (Casp3), and levels of released inflammatory cytokines, including IL-4 and IL-6, were monitored in response to the chemical treatment.
Results: Our findings showed insufficient inhibition of the indicated factors by IFN-γ and rapamycin in cancer cells when compared to Sorafenib. Interestingly, vitamin B17 revealed competitive inhibition on cell proliferation of HepG2 cells compared with Sorafenib while in stem cells, vitamin B17 led to impartial consequences. Unlike TP53 and caspase 3, gene expressions of Raf-1 and LC3B were significantly reduced in cancer cells treated with vitamin B17 at both RNA and protein levels while their expression was markedly upregulated in the treated stem cells. Furthermore, in both cells, vitamin B17 increased the expression of IL-4 while reducing the production of IL-6.
Conclusion: These data provide evidence for the effectiveness of vitamin B17 in cancer treatment via selective regulation of Raf-1 and autophagy-related LC3B in cancer cells.

Keywords

This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination, and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi:10.30476/mejc.2022.93036.1673

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