Document Type : Original Article(s)
Authors
- Habib Zarredar 1
- Milad Asadi 2
- Dariush Shanehbandi 3
- Venus Zafari 2
- Soghra Bornodeli 3
- Zahra Soleimani 1
- Shahram Ghasembaglou 1
1 Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2 Department of Basic Oncology, Ege University, Institute of Health Sciences, Izmir, Turkey
3 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract
Background: Long noncoding RNA (lncRNA) is recognized as an essential controller of gene expression and other activities of the cells. Additionally, lncRNAs have a critical role in the progression and growth of human malignancies, like melanoma. Among lncRNAs, thymopoietin (TMPO)-antisense RNA 1 (TMPO AS- 1) has a significant role in melanoma. The current study aimed to determine the expression level of TMPO AS-1 in melanoma patients.
Method: In this case-control research, 50 pairs of tumor and non-tumor tissues of melanoma patients were separated by the surgeon. Subsequently, TMPO AS-1 expression level in the tissues was evaluated. We used TRIzol to extract total RNA from the tumor and non-tumor tissues, following which complementary DNA was synthesized. The TMPO AS-1 expression level of TMPO AS-1 was evaluated via quantitative reverse transcription- polymerase chain reaction (qRT-PCR) technique. Moreover, clinicopathological features of the melanoma patients were evaluated.
Results: Our findings revealed that TMPO AS-1 expression level was upregulated in the melanoma tissues in comparison with the non-tumor ones. Remarkably, the TMPO AS-1 expression level was considerably correlated to the clinicopathological characteristics of the patients, including lymph nodes and distant metastasis.
Conclusion: Upregulation of TMPO AS-1 in melanoma indicated that TMPO AS- 1 and its downstream signal pathways could be used as a new target treatment option and prognostic tumor marker for melanoma.
Highlights
Keywords
How to cite this article:
Zarredar H, Asadi M, Shanehbandi D, Zafari V, Bornodeli S, Soleimani Z, et al. Evaluation of the LncRNA TMPO AS-1 expression level in melanoma tissues in comparison to paired normal marginal tissues. Middle East J Cancer. 2023;14(3):399-404. doi: 10.30476/mejc.2022. 94761.1744.
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