Document Type : Original Article

Authors

1 Department of Radiation Sciences, Medical Research Institute, Alexandria University, Alexandria, Egypt

2 Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria, Egypt

3 Department of Applied Medical Chemistry, Medical Research Institute, Alexandria University, Alexandria, Egypt

4 Department of Pathology, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt

5 Medical Physics and Radiotherapy Department, Ayadi Almostakbal Oncology Center, Alexandria, Egypt

10.30476/mejc.2021.86945.1377

Abstract

Background: We aimed to investigate the antitumor potentiality and radiosensitizing effect of Fagonia arabica ethanolic extract (FAEE) on mice bearing solid ehrlich carcinoma (EC).
Method: This experimental animal study included 90 Balb-c mice and divided them into four groups: Group I: 10 EC-bearing mice as untreated controls; Group II: 10 EC-bearing mice exposed to a single dose of ionizing radiation (IR) at tumor localization (6Gy); Group III: 30 EC-bearing mice, each 10 mice received different dose of FAEE (250, 500, and 1000 mg/kg/day); Group IV: 30 EC-bearing mice, each 10 mice received different dose of FAEE (250, 500, and 1000 mg/kg/day) plus a single dose of IR (6Gy). P-FOXO3a and p-AKT levels were measured in tumor tissue homogenate via ELISA technique. Bcl-2 gene expression was assessed with RT-PCR. Tumor tissues were stained with Haematoxylin and Eosin stain and examined.
Results: FAEE has antiproliferative effect on EC-bearing mice reflected by the decrease in tumor volume and tumor growth rate in a dose-dependent manner. Combination of FAEE with IR significantly increased radiation-induced tumor damage in comparison with IR alone. We observed a significant decrease in the concentration of p-AKT and p-FOXO3a and down-regulation of Bcl-2 gene in the EC-bearing mice treated with FAEE only or in combination with IR.
Conclusion: FAEE may be an effective anti-tumor agent against breast cancer. FAEE exerts radio-sensitizing effect, especially at a dose of 500 mg/kg. FAEE interferes with the apoptosis process via decreasing p-AKT and p-FOXO3a and down-regulation of Bcl-2 gene.

Keywords

This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination, and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi:10.30476/mejc.2021.86945.1377