Document Type : Original Article

Authors

Department of Clinical Biochemistry, Medicine school, Hamadan University of Medical Sciences, Hamadan, Iran

10.30476/mejc.2021.92306.1653

Abstract

Background: Thymoquinone (TQ), an active part of Nigella sativa, has been reported as an anti-cancer agent. This study aimed to evaluate different anti-cancer effects of TQ on oxidative stress markers and Peroxiredoxin4 (P4) in lung cancer A549 cell line.
Method: In this experimental study, we used TQ concentrations to treat lung A549 cells and determined cell viability by the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay at 12h, 24h, and 48h times. The IC50 concentration of TQ was found with MTT assay. We studied the total antioxidant capacity (TAC) and total oxidant status (TOS) using the manual assay, and analyzed catalase (CAT), superoxide dismutases (SOD), and glutathione peroxidase (GPx) activity using the enzyme-linked immunoassay (ELISA) kits. Moreover, the concentration of peroxiredoxin-4 (PRXD4) was measured with the ELISA Kit.
Results: The IC50 of TQ for A549 cells was calculated to be 40 µM concentration for 24 h of incubation. TAC index significantly decreased in the treated cells compared to the controls (P = 0.05) whereas TOS and PRXD4 levels showed a significant increase (P = 0.05). Additionally, the results showed that the CAT, SOD, and GPX activity enzymes significantly decreased in 20, 40, and 60 µM TQ in comparison with the control cells (P = 0.05).
Conclusion: TQ has significant inhibitory effects on A549 cells and could be utilized in novel therapy not only for lung cancer, but also for other tumors.

Keywords

This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination, and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi:10.30476/mejc.2021.92306.1653