Document Type: Original Article

Authors

1 National Institute of Cancer Research and Hospital, Mohakhali, Dhaka, Bangladesh

2 Genetic Engineering and Biotechnology Research Centre for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka, Bangladesh

3 Department of Surgery, East West Medical College, Uttara, Dhaka, Bangladesh

4 Department of Surgical Oncology, Khulna Medical College, Khulna, Bangladesh

5 Department of Gastroenterology, Popular Medical College Hospital, Dhanmondi, Dhaka, Bangladesh

6 Department of Gastroenterology, Khulna Medical College, Khulna, Bangladesh

Abstract

Background: Gene alterations are required for the development of gastric cancer, which are influenced by environmental and host factors. We conducted the present study to find the status of Helicobacter (H) pylori infection and its association with altered genes P53, hMLH1, and HER2 in gastric cancer patients and to analyse their correlation with clinical, pathological, and environmental factors.
Method: This was a cross-sectional study. For genetic (P53 and hMLH1) study of the gastrectomized tissue DNA extraction and optimization, we performed PCR amplification and DNA sequencing. HER2 was studied by immunochemical technique. The results were associated with tumor status, age and sex, smoking, and H pylori antibody status of the patients.
Results: The mean age of the patients was 52.91 (±13.94) years. Among the 45 patients selected for genetic tests, 12 aged 40 or more and 33 aged over 40. Among the genes, 33(73.3%) in p53 and 17(37.7%) in hMLH1 were mutated, and 11(24.2%) in HER2 were found to be overexpressed. Chi square and regression analysis showed that they all had associations with H pylori positivity (p value <.05, odd ratio >1). hMLH1 was associated with the location of the tumor, smoking, sex, blood group, and age, and p53 was found to be affected by extra salt intake, sex, blood group, and age of the patients (P≤.05 ).
Conclusion: Genetic mutation was found in nearly all the patients with gastric cancer, which was significantly associated with H pylori infection. Mass eradication of this organism might play a role in reducing cancer incidence in Bangladesh.

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This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination, and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.30476/mejc.2020.84147.1207