Document Type: Original Article

Authors

1 Department of Hematology and Blood Banking, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran

2 Department of Pharmacognosy and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Background: Amygdalin is a glycoside ingredient of rosacea plants that exerts an antitumor effect by blocking the growth of the tumor cells. Therefore, we aimed to use the Armeniacae semen, a member of the Rosacea family, which contains a large amount of the amygdalin, to evaluate its anti-proliferative effect on MCF-7, MDA-MB-231, and T47D breast cancer cell lines.
Method: In this experimental study, we prepared the aqueous, ethyl acetate, and hydro alcoholic extracts of the Armeniacae semen. The MCF-7, MDA-MB-231, and T47D cell lines were treated with different doses of the extracts for 12, 24, 36, and 48 hours; cell viability was investigated with MTT test, and cell apoptosis was detected by use of double staining fluorescent. Cell cycle progression was analyzed using a BD Cycle TEST PLUS DNA Kit. We also assessed Bcl2, Bax, and caspase-3 mRNA expression.
Results: The best IC50s belonged to Hydro alcoholic extract of the Armeniacae semen in all three cell lines for the 48-hour treatment. We observed a significant increase in Bax and caspase-3 mRNA expression and a noticeable reduction in Bcl2 mRNA compared to the controls. Application of amygdalin to MCF-7, MDA-MB-231, and T47D cell lines increased the number of G0/G1 cells and reduced the number of cells at G2/M phasecompared to the controls.
Conclusion: This study showed that the hydro alcoholic extract of Prunus armeniaca had antitumor effects on breast cancer cell lines as it inhibited the cell cycle at G0/G1 phase and apoptosis induction in the MCF-7, MDA-MB-231, and T47D cell lines.

Keywords

This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination, and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi:10.30476/mejc.2020.83374.1159