Evaluation of an Immunomodulator Drug as a Radioprotectant on Human Peripheral Blood Lymphocytes In Vitro

Sattarpour, Zahra; Baradaran, Bahzad; Farajollahi, Alireza; Asghari Jafarabadi, Mohammad; Khazeh, Vahid; Pirayesh Islamian, Jalil

doi:10.30476/mejc.2018.42102

Evaluation of an Immunomodulator Drug as a Radioprotectant on Human Peripheral Blood Lymphocytes In Vitro

Document Type : Original Article(s)

Authors

Zahra Sattarpour ¹

Bahzad Baradaran ¹

Alireza Farajollahi ²

Mohammad Asghari Jafarabadi ³

Vahid Khazeh ¹

Jalil Pirayesh Islamian ²

¹ Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

² Department of Medical Physics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

³ Department of Statistics and Epidemiology, Faculty of Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran

https://doi.org/10.30476/mejc.2018.42102

Abstract

Background: IMOD™, a selenium enriched extract of the plants Tanacetum vulgare, Urtica dioica, and Rosa canina, has an excellent effect on oxidative stress. In this study, we investigated the radioprotective effects of this immunomodulatory drug on human peripheral blood lymphocytes.Methods: Peripheral blood samples obtained from venipuncture of the brachial vein were treated with IMOD™ (5, 10, 15, 20 μl) for 30 min and Cobalt 60 γ-rays (0.25, 0.5, 1, 2 Gy) as the test groups and cultured with the control. We used the micronuclei assay, cell death detection, and cell toxicity assay to analyze the treatment effects.Results: The frequency of micronuclei were 1.66 (0 Gy), 5.33 (0.25 Gy), 9.67 (0.5 Gy), 17.67 (1 Gy), and 23.67 (2 Gy) in the irradiated lymphocytes (P<0.001). The percentage of micronuclei frequency reduced to 20%, 26.83%, 37.68%, 16%, and 20.47% with IMOD™. Apoptosis and necrosis decreased significantly in the IMOD™ treated groups (P<0.05).Conclusion: IMOD™ may protect these cells against ionizing radiation.