Shiraz University of Medical Sciences
Middle East Journal of Cancer
2008-6709
2008-6687
8
3
2017
07
01
Evaluation of the Cytotoxic Effects of Ciprofloxacin on Human Glioblastoma A-172 Cell Line
119
126
EN
Ashkan
Zandi
Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical
Sciences, Tehran, Iran
dr.ashkan.zandi@gmail.com
Taraneh
Moini Zanjani
Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical
Sciences, Tehran, Iran
t.moini@sbmu.ac.ir
Seyed Ali
Ziai
Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical
Sciences, Tehran, Iran
aliziai@sbmu.ac.ir
Yalda
Khazaei Poul
Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical
Sciences, Tehran, Iran
kazaei_yalda@yahoo.com
Mostafa
Haji Molla Hoseini
Department of Immunology, School of Medicine, Shahid Beheshti University of Medical
Sciences, Tehran, Iran
m.mollahoseini@sbmu.ac.ir
Background: Glioblastoma multiforme, the most common, aggressive malignant brain tumor which affects patients of all ages, is principally resistant to treatment. Ciprofloxacin is an antibiotic that belongs to the fluoroquinolones. There are welldocumented observations which indicate that ciprofloxacin has substantial anti-proliferative, apoptotic, cytotoxic and oxidative stress activities on various tumor cell lines.Methods: We exposed the glioblastoma A-172 cell line to ciprofloxacin for 24, 48 and 72 h. Cytotoxicity was measured using MTT assay. The levels of Bax as an apoptotic and Bcl-2 as an anti-apoptotic protein were measured by ELISA and oxidative stress by the malondialdehyde assay.Results: Ciprofloxacin induced tumor cell death in a dose-dependent manner with an IC50 value of 259.3 μM at 72 h. We observed an increase in Bax levels, a decrease in Bcl-2 concentrations and increased Bax/Bcl-2 ratio under the influence of ciprofloxacin. Malondialdehyde levels, as an important marker of oxidative stress, increased in the human glioblastoma A-172 cell line.Conclusion: These results indicated that ciprofloxacin had anti-tumor, cytotoxic and apoptotic effects in the human glioblastoma A-172 cell line which might be useful as an adjuvant added to a glioblastoma multiforme chemotherapeutic protocol in the future.
https://mejc.sums.ac.ir/article_42078.html
https://mejc.sums.ac.ir/article_42078_a46cd593ec34ec5c87ad24f1a74bbbcb.pdf
Shiraz University of Medical Sciences
Middle East Journal of Cancer
2008-6709
2008-6687
8
3
2017
07
01
Neoadjuvant Chemotherapy and Surgical Options for Locally-advanced Breast Cancer: A Single Institution Experience
127
134
EN
Mohamed
Abo Elmagd
Salem
Department of Surgical Oncology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt
m.salem@aun.edu.eg
Hamza
Abbas
Hamza
Department of Radiation Therapy, South Egypt Cancer Institute, Assiut University, Assiut, Egypt
hamza_assiut@yahoo.com
Nashwa Mohamed
Abd El-Raouf Abd El-Aziz
Department of Medical Oncology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt
nashwa_raouf@yahoo.com
Background: Neoadjuvant chemotherapy can downstage the size of the tumor, thus allowing some patients with advanced disease with the option of conservative breast surgery. Our study aims to investigate the effectiveness of neoadjuvant chemotherapy in patients with locally advanced breast cancer.Methods: Fifty-six patients had locally advanced breast cancer. Ten patients (18%) were stage IIB, 32 (57%) were stage IIIA, 9 (16%) were stage IIIB, and 5 (9%) were stage IIIC. Patients received neoadjuvant chemotherapy comprised of cyclophosphamide, doxorubicin, and fluorouracil followed by surgery (15 patients with breast conservative surgery,11 with skin sparing mastectomy and latesmus dorsi reconstruction, and 30 patients who underwent modified radical mastectomy) and then followed by radiotherapy, 50 Gy with conventional fractionation.Results: Clinical down staging was obtained in 49 (87.5%) patients: 5 (9%) had complete clinical response, 44 (78.5%) had partial response, 6 (10.7%) had stable disease, and 1 (1.8%) had progressive disease. The primary tumor could not be palpated after chemotherapy in 7 (12.5%) of 56 patients who presented with a palpable mass. Median follow-up was 47.5 months. The factors that correlated positively with locoregional recurrence on univariate analysis included hormonal receptor status and surgical margin status. On multivariate analysis, surgical margin status was the only independent significant factor for locoregional recurrence-free survival. In univariate analysis for distant relapse free survival, factors that correlated positively included disease stage and hormonal receptor status. Multivariate analysis showed that tumor stage and hormonal receptor status were independent significant factors that correlated with distant relapse-free survival.Conclusion: Neoadjuvant chemotherapy was effective in clinical down staging and should be considered for patients with advanced breast cancer. It improved operability and enhanced local control and increased the possibility of breast-conserving surgery without affecting overall survival. Negative surgical margin was the independent significant factor in terms of locoregional recurrence while tumor stage and hormonal receptor status were the independent significant factors in term of distant relapse free survival.
https://mejc.sums.ac.ir/article_42079.html
https://mejc.sums.ac.ir/article_42079_91df87e87233b4215c1e227401af8388.pdf
Shiraz University of Medical Sciences
Middle East Journal of Cancer
2008-6709
2008-6687
8
3
2017
07
01
Predictors of Early Radiation Induced Esophageal Toxicity in Radiotherapy of Locally Advanced Non-small Cell Lung Cancer
135
141
EN
Maher
Soliman
0000-0002-4811-271X
Oncology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt
maher.soliman@daad-alumni.de
Background: Radiation induced esophageal toxicity is a primary cause of treatment interruptions in the radiotherapy of lung cancer, for which there are no clear predictive factors. This study attempts to identify risk factors associated with the development of severe radiation induced esophageal toxicity using clinical and dosimetric parameters.Methods: We reviewed the medical records of 54 patients with histologically proven stage III non-small cell lung cancer treated with 3D-conformal radiotherapy at Alexandria Main University Hospital between January 2008 and December 2011. The original treatment plans for those patients were restored and imported to the treatment planning system. The external surface of esophagus was contoured for each patient. We calculated the esophagus dose–volume histograms and various dosimetric parameters. Univariate and multivariate logistic regression analyses were performed.Results: Of the 54 patients, 6 (11.1%) had grade 3 radiation induced esophageal toxicity and 2 (3.7%) had grade 4. There was no grade 5 toxicity. The most statistically significant parameters for predicting RIET grade 3 or worse included esophageal volume that received ≥50 Gy (V50), esophageal volume that received ≥55 Gy (V55), and the use of concurrent chemotherapy according to univariate and multivariate logistic regression analyses.Conclusion: This study demonstrates that the best predictive factors for severe early radiation induced esophageal toxicity are concurrent chemotherapy, and esophageal volumes ≥50 Gy and ≥55 Gy in non-small cell lung cancer treated with 3D-conformal radiotherapy.
https://mejc.sums.ac.ir/article_42080.html
https://mejc.sums.ac.ir/article_42080_4c6ed565f234b48932446fcf15c8f41b.pdf
Shiraz University of Medical Sciences
Middle East Journal of Cancer
2008-6709
2008-6687
8
3
2017
07
01
Wilms’ Tumor: Histopathological Variants and the Outcomes of 31 Cases at a Tertiary Care Center in Northern India
143
150
EN
Pradeep
Kajal
Department of Pediatric Surgery, PGIMS Rohtak, Haryana, India
drpradeepkajal@gmail.com
Kamal
Nain
Rattan
Department of Pediatric Surgery, PGIMS Rohtak, Haryana, India
knrattan@gmail.com
Namita
Bhutani
0000-0003-2436-9016
Department of Pathology, PGIMS Rohtak, Haryana, India
namitabhutani89@gmail.com
Nisha
Marwah
Department of Pathology, PGIMS Rohtak, Haryana, India
nmarwah@gmail.com
Background: Wilms’ tumor is the most common malignant renal tumor in the pediatric age group. This tumor is classically managed by multimodal treatment which involves surgery, radiotherapy and chemotherapy. While there is plenty of data in world literature on the outcome of Wilms’ tumor, there is a paucity of data from India.Methods: All patients with proven diagnosis of Wilms’ tumor between 2008 to 2012 were noted from the hospital’s cancer registry. We performed detailed analyses of all patients’ clinical case records for demographic profiles, clinical features, imaging studies, treatment, and outcome. Histopathological classification of the tumor determined the patient’s post-operative management. All patients were followed for a period of 3 years and we analyzed the eventual outcome in the form of disease-free survival, complications, tumor recurrence, and mortality.Results: There were 31 cases of Wilms’ tumor included in this study. The median age of presentation was 3-4 years (range: 5 months-6 years) with a female: male ratio of 1.2:1. Abdominal mass was the chief presenting feature in 20 (64.5%) patients followed by abdominal pain in 6 (19.3%). All children had unilateral disease, 25 (80.6%) had right-sided and 6 (19.3%) had left-sided disease. Bilateral disease was seen in only one case. Of the 31 cases of Wilms’ tumor, 36% cases presented with stages I and II disease, 55% had stage III, and 9% of the cases were stage IV. Most cases of Wilms’ tumor were stage III. The majority had classical Wilms’ tumor with a favourable histology. The estimated 5-year event free survival was 87.3%Conclusion: A multidisciplinary approach can approach similar survival rates compared to the National Wilms’ Tumor Study Group, even in the Indian scenario. Further improvement in survival of these children can only be achieved by increasing awareness, early recognition, appropriate referral, and a multidisciplinary approach.
https://mejc.sums.ac.ir/article_42081.html
https://mejc.sums.ac.ir/article_42081_b0fddfcf186be46c9fdcaeca6253f57f.pdf
Shiraz University of Medical Sciences
Middle East Journal of Cancer
2008-6709
2008-6687
8
3
2017
07
01
Sexual Function in Cervical Cancer Survivors after Concurrent Chemoradiotherapy
151
154
EN
Dhiraj
Daga
Department of Radiotherapy, RUHS College of Medical Sciences, RDBP Jaipuria
Hospital, Jaipur, Rajasthan, India
dhirajdaga@gmail.com
Rohitashwa
Dana
Department of Radiotherapy, SMS Medical College and Attached Hospitals, Jaipur,
Rajasthan, India
rdana2000@yahoo.co.in
Pradeep
Gaur
Department of Radiotherapy, Dr S.N.Medical College, Mathura Das Mathur Hospital,
Jodhpur, Rajasthan, India
b4jodhpur@gmail.com
R.K.
Spartacus
Department of Radiotherapy, SMS Medical College and Attached Hospitals, Jaipur,
Rajasthan, India
meg80224@gmail.com
Background: This study evaluated sexual function in cervical cancer survivors after concurrent chemoradiotherapy.Methods: Study participants comprised survivors of locally advanced cervical cancer (stages IIB-IVA) who completed concurrent chemoradiotherapy along with intracavitary brachytherapy at least two years prior at Dr S.N.Medical College, Jodhpur, Rajasthan, India. We used the Female Sexual Function Index questionnaire to assess sexual function. The cut-off score of the Female Sexual Function Index that identified female sexual arousal disorder was 26.55. A score less than 26.55 indicated the presence of female sexual arousal disorder.Results: A total of 48 locally advanced cervical cancer survivors enrolled in the study. Survivors had a mean age of 46.5 years. All received chemoradiotherapy along with intracavitary brachytherapy. The average time for treatment was 53.5 days. Patients had an average score for sexual desire of 2, 2.3 for arousal, 2.3 for sexual satisfaction, and 2.1 for pain during intercourse. The overall average score was 11.84 (range: 3.2-19.5) with a cut-off of 26.55. All survivors suffered from female sexual arousal disorder.Conclusion: Cervical cancer survivors had decreased sexual function which indicated female sexual arousal disorder. Patient education and active treatment of complications related to cancer treatments is a must for improvement of sexual function among survivors. Long-term complications should be considered in terms of treatment planning and follow-up treatment to improve the quality of life of cancer survivors.
https://mejc.sums.ac.ir/article_42082.html
https://mejc.sums.ac.ir/article_42082_2c60a8a9a944f44921ab97fdad50b642.pdf
Shiraz University of Medical Sciences
Middle East Journal of Cancer
2008-6709
2008-6687
8
3
2017
07
01
Diagnostic Accuracy of Intraoperative Frozen Section and Causes of Error in Ovarian Epithelial Tumors: An Institutional Experience
155
160
EN
Fatemeh
Nili
Department of Pathology, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, I.R. Iran
f_nili@yahoo.com
Vahid
Soleimani
Department of Pathology, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, I.R. Iran
Behnaz
Jahanbin
Department of Pathology, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, I.R. Iran
b_jahanbin@yahoo.com
Seyed Siamak
Jalal-Alhoseini
Department of Pathology, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, I.R. Iran
siyamakj@yahoo.com
Alireza
Ghanadan
Department of Pathology, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, I.R. Iran
dermpath101@gmail.com
Background: Ovarian cancer is the second most common type of female genital tract malignancy. Treatment planning differs for benign, borderline, and malignant subtypes of surface epithelial tumors and depends on accurate histopathological diagnosis. The aim of this study is to determine the accuracy and causes for error in intraoperative diagnosis of ovarian surface epithelial tumors.Methods: In this retrospective study, we analyzed all cases of ovarian surface epithelial tumors referred to the Pathology Department of our hospital from April 2010 to December 2015. We considered the final diagnosis as the gold standard and determined the accuracy, sensitivity, specificity, positive predictive value, negative predictive value, underdiagnosis and overdiagnosis for each group of benign, borderline, and malignant tumors. An expert pathologist blinded to the diagnosis reviewed patients’ frozen and permanent slides and categorized causes of error into misinterpretation, sampling, and technical errors.Results: We assessed 220 patients’ slides (96 benign, 66 borderline, and 58 malignant tumors). The accuracy of the frozen section was: 98% in benign, 80.3% in borderline and 67.2% in malignant tumors. The frozen sections had a sensitivity of 97.9% for benign tumors and a sensitivity of 67.2% and specificity of 100% for malignant tumors. Borderline tumors had a sensitivity of 91% and specificity of 88.4%. Mucinous borderline tumors comprised the more frequent uncertain and underdiagnosed cases. The main cause for error in this group was sampling error. In malignant neoplasms, 15.5% were reported to be at least borderline. Technical issues were the cause of difficulty in interpretation. In the benign category, cystadenofibroma could be misinterpreted as a borderline malignancy.Conclusion: Frozen section is an accurate, specific method for diagnosis of benign and malignant tumors. In the borderline category, the results should be interpreted with caution.
https://mejc.sums.ac.ir/article_42083.html
https://mejc.sums.ac.ir/article_42083_b3a87549fa69b1cac557e78f6effe620.pdf
Shiraz University of Medical Sciences
Middle East Journal of Cancer
2008-6709
2008-6687
8
3
2017
07
01
Small Cell Carcinoma of the Ovary: Report of a Case with Unusual and Aggressive Presentation
161
165
EN
Fatemeh Sadat
Najib
Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
najibf@sums.ac.ir
Mojdeh
Momtahan
Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Maral
Mokhtari
0000-0002-4815-2631
Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
maral_mokhtari@yahoo.com
Shaghayegh
Moradi Alamdarloo
Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
shaghayeghmoradi84@gmail.com
Tahereh
Poordast
Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
ta.poordast@yahoo.com
Small cell carcinoma of the ovary is an aggressive malignant tumor with no standard treatment. Despite surgery, chemotherapy and radiation, this tumor has a poor prognosis with rapid progression. The authors report a case of small cell carcinoma of the ovary in a 37-year-old woman who presented twice with an acute abdomen and unstable hemodynamics which led to two urgent laparatomies. The patient died two months after her diagnosis of small cell carcinoma of the ovary and one course of chemotherapy.
https://mejc.sums.ac.ir/article_42077.html
https://mejc.sums.ac.ir/article_42077_02fc779e7eb2ca097da237052531098d.pdf
Shiraz University of Medical Sciences
Middle East Journal of Cancer
2008-6709
2008-6687
8
3
2017
07
01
A Child with Left Femoral Pain and Limping
167
169
EN
Nader
Shakibazad
0000-0002-5124-6380
Department of Pediatrics, Hematology-Oncology Branch, Shiraz University of Medical
Sciences, Shiraz, Iran
nshakibazad@gmail.com
Mohammadreza
Bordbar
0000-0002-8071-6425
Department of Pediatrics, Hematology-Oncology Branch, Shiraz University of Medical
Sciences, Shiraz, Iran
mbordbar53@gmail.com
Mahdi
Shahriari
0000-0001-5104-9590
Department of Pediatrics, Hematology-Oncology Branch, Shiraz University of Medical
Sciences, Shiraz, Iran
shahryar@sums.ac.ir
https://mejc.sums.ac.ir/article_42076.html
https://mejc.sums.ac.ir/article_42076_1cdb5f588a344fc149f1d9a34cbc394a.pdf
Shiraz University of Medical Sciences
Middle East Journal of Cancer
2008-6709
2008-6687
8
3
2017
07
01
Calendar of Events
170
170
EN
10.30476/mejc.2019.81459.1013
https://mejc.sums.ac.ir/article_44733.html
https://mejc.sums.ac.ir/article_44733_75c15bfb9bfd96f60edeaa01078d0c51.pdf