%0 Journal Article %T ST6Gal1, Cox-2 and HB-EGF mRNA Expression in Breast Cancer Samples from Kashan, Iran %J Middle East Journal of Cancer %I Shiraz University of Medical Sciences %Z 2008-6709 %A Taherian, Aliakbar %A Mostafavi Zadeh, Seyed Mostafa %A Ghani, Hossein %A Khamechian, Tahere %D 2015 %\ 01/01/2015 %V 6 %N 1 %P 43-50 %! ST6Gal1, Cox-2 and HB-EGF mRNA Expression in Breast Cancer Samples from Kashan, Iran %R %X Background: ST6Gal1, Cox-2 and HB-EGF genes are involved in different tumors and their enhanced expressions often correlate with poor prognosis. In this study we assay the expressions of these genes by reverse transcriptase-PCR in 54 breast cancer samples.Methods: Tissue samples were either formalin-fixed for histopathological examination or frozen for reverse transcriptase-PCR. Image program was used for the densitometry of the image of the gels and the expression of different genes was normalized with beta actin expression. The student's t-test and correlation matrix were used for data analyses.Results: We observed significantly higher expressions of ST6Gal1 (P= 0.040), Cox- 2 (P= 0.001) and HB-EGF (P= 0.009) in the tumor region compared to the margin samples. A significant correlation was found between HB-EGF and Cox-2 expression (P= 0.001). There was a positive correlation between total score, tumor size, histology grade and nuclear grade but there was a reverse correlation between age and tumor size, histology grade and total score. Conclusion: Expressions of ST6Gal1, Cox-2 and HB-EGF in breast tumor samples in this and a number of other studies emphasize their role as important markers in breast cancer . The use of medications to inhibit either their individual expressions or the possible inhibition of all three genes may improve patient survival and prevent metastasis. %U https://mejc.sums.ac.ir/article_41987_a906c439f5fae13c09755718106957f0.pdf