@article { author = {Mahdizade Valojerdi, Fereshte and Goliaei, Bahram and Rezakhani, Nakisa and Nikoofar, Alireza and Keshmiri Neghab, Hoda and Soheilifar, Mohammad Hasan and Bigdeli, Bahareh}, title = {In Vitro Radiosensitization of T47D and MDA-MB-231 Breast Cancer Cells with the Neoflavonoid Dalbergin}, journal = {Middle East Journal of Cancer}, volume = {14}, number = {2}, pages = {205-218}, year = {2023}, publisher = {Shiraz University of Medical Sciences}, issn = {2008-6709}, eissn = {2008-6687}, doi = {10.30476/mejc.2022.91913.1637}, abstract = {Background: Radiotherapy is a frequently used therapeutic modality for breast cancer. Dalbergin, a natural antioxidant, inhibits carcinogens and tumor progression. In the present study, we investigated the effect of Dalbergin on the response of T47D and MDA-MB-231 breast cancer cell lines to ionizing radiation.Method: In this experimental in vitro study, doubling time of T47D and MDAMB- 231 were obtained from the growth curve. The cytotoxic effect of Dalbergin on T47D and MDA-MB-231 breast cancer cells were estimated via MTT assay. To determine the clonogenic ability, we treated T47D and MDA-MB-231 with Dalbergin for 48 h prior to irradiation, subsequent to which a colony assay was performed. Real-time polymerase chain reaction was employed to determine the gene expression level.Results: Dalbergin inhibited proliferation of T47D and MDA-MB-231 in a time and concentration-dependent manner. Additionally, the most appropriate time for the treatment of these types of cancer cells was found to be 48 h and the drug's concentration in both cell lines was different. The IC50 values of T47D and MDA-MB-231 cells were 0.001 and 0.0001 μM, respectively. Moreover, this drug radiaosensitizes both cell lines effectively compared with the radiation only. Finally, the gene expression level of p53, Bcl-2, and STAT3 were investigated in cancer cells.Conclusion: Dalbergin showed apoptotic effects probably through the STAT/p53 signaling pathway. Therefore, Dalbergin could be considered as a radiosensitizer and its effects may be owing to increased cell death.}, keywords = {Dalbergin,Cells,X-rays,Apoptotic,Cell death}, url = {https://mejc.sums.ac.ir/article_48305.html}, eprint = {https://mejc.sums.ac.ir/article_48305_248ccf0d669127c71bad3dbc754a61ac.pdf} }