@article { author = {Safaei, Sahar and Shanehbandi, Dariush and Zafari, Venus and Eghbali, Elham and Sadeghzadeh, Mahsa and Mohammad Reza Khani, Haniye and Sadrazar, Amin and Faghihdinevari, Masood and Shirmohamadi, Masoud}, title = {Evaluation of miR-107, DAPK1, and KLF4 Expression in Colorectal Tumors and Effect of Oxaliplatin and 5-FU on their Levels in Colorectal Cancer Cell Lines}, journal = {Middle East Journal of Cancer}, volume = {12}, number = {2}, pages = {190-197}, year = {2021}, publisher = {Shiraz University of Medical Sciences}, issn = {2008-6709}, eissn = {2008-6687}, doi = {10.30476/mejc.2020.83091.1131}, abstract = {Background: In recent years, the role of micro-RNAs in the cancer pathophysiology has attracted a great deal of scientific attention. MiRNAs regulate a variety of cellular functions, such as apoptosis, differentiation and migration by targeting oncogenic or tumor suppressor genes. We conducted the current study to assess the expression of miR-107, Krüppel-like factor 4 (KLF4) and death-associated protein kinase (DAPK1) genes in malignant and normal colon tissues and also colorectal cancer (CRC) model cells exposed to oxaliplatin and 5-FU chemotherapy agents. Method: In this case-control study, the tissue samples from CRC patients were collected during colonoscopy process in 2013 -2016 at Imam Reza hospital. Subsequently, the expression levels of miR-107, KLF4, and DAPK1 were detected with quantitative Real-Time PCR. Furthermore, in the in vitro phase of this study, we investigated the changes in the expression level of miR-107, KLF4 and DAPK1 transcripts after oxaliplatin and 5-FU treatment. Results: Unlike miR-107, the expression levels of KLF4 and DAPK1 genes decreased in the tumor samples compared to those in the marginal specimens. In addition, both oxaliplatin and 5-FU significantly increased the expression level of miR-107. There were significant correlations between the expression levels of miR-107, KLF4, and DAPK1genes and clinicopathological features, for instance lymph node metastasis and cell differentiation. Conclusion: The current study suggested a tumor suppressor role for KLF4 and DAPK1 in CRC. The altered expression of miR-107, KLF-4, and DAPK1 genes in CRC tumors and healthy tissues could be utilized for CRC diagnosis and prognosis. Furthermore, the studied genes could be considered as potential therapeutic targets in CRC.}, keywords = {Colorectal neoplasms,miR-107,KLF4,DAPK1,Oxaliplatin,5-Fu}, url = {https://mejc.sums.ac.ir/article_47019.html}, eprint = {https://mejc.sums.ac.ir/article_47019_d73420a2240ab48967efe3e2555333e3.pdf} }