@article { author = {Taherian, Aliakbar and Mostafavi Zadeh, Seyed Mostafa and Ghani, Hossein and Khamechian, Tahere}, title = {ST6Gal1, Cox-2 and HB-EGF mRNA Expression in Breast Cancer Samples from Kashan, Iran}, journal = {Middle East Journal of Cancer}, volume = {6}, number = {1}, pages = {43-50}, year = {2015}, publisher = {Shiraz University of Medical Sciences}, issn = {2008-6709}, eissn = {2008-6687}, doi = {}, abstract = {Background: ST6Gal1, Cox-2 and HB-EGF genes are involved in different tumors and their enhanced expressions often correlate with poor prognosis. In this study we assay the expressions of these genes by reverse transcriptase-PCR in 54 breast cancer samples.Methods: Tissue samples were either formalin-fixed for histopathological examination or frozen for reverse transcriptase-PCR. Image program was used for the densitometry of the image of the gels and the expression of different genes was normalized with beta actin expression. The student's t-test and correlation matrix were used for data analyses.Results: We observed significantly higher expressions of ST6Gal1 (P= 0.040), Cox- 2 (P= 0.001) and HB-EGF (P= 0.009) in the tumor region compared to the margin samples. A significant correlation was found between HB-EGF and Cox-2 expression (P= 0.001). There was a positive correlation between total score, tumor size, histology grade and nuclear grade but there was a reverse correlation between age and tumor size, histology grade and total score. Conclusion: Expressions of ST6Gal1, Cox-2 and HB-EGF in breast tumor samples in this and a number of other studies emphasize their role as important markers in breast cancer . The use of medications to inhibit either their individual expressions or the possible inhibition of all three genes may improve patient survival and prevent metastasis.}, keywords = {}, url = {https://mejc.sums.ac.ir/article_41987.html}, eprint = {https://mejc.sums.ac.ir/article_41987_a906c439f5fae13c09755718106957f0.pdf} }